Herpes simplex virus (HSV) is one of the most common human pathogens. Indirect enzyme-linked immunosorbent assay The study indicates that gG 321–580His has a high diagnostic potential for HSV-2 virus serodiagnosis in humans. In testing 318 field serum samples, the diagnostic relative sensitivity and specificity of the developed gG 321–580His-ELISA test in qualitative comparison with the commercial kit were 93.81% and 96.74%, respectively, and the accuracy was 94.65%. An indirect enzyme-linked immunosorbent assay (ELISA) was developed using gG 321–580His as the diagnostic antigen and evaluated by comparison with a commercial HerpeSelect 2 ELISA immunoglobulin G kit as reference. We successfully expressed a fragment of gG comprising residues 321–580 of HSV-2 with histidine tag (gG 321–580His) in a Bac-to-Bac baculovirus expression system, which had an antigenicity similar to its native counterpart. As immunological diagnosis kits for accurate differentiation between HSV-1 and HSV-2 antibodies can be expensive, there is a need to develop a convenient, sensitive, specific and cost-effective serodiagnostic kit. Glycoprotein G (gG) is a prototype antigen for type-specific serodiagnosis distinguishing between HSV type 1 (HSV-1) and HSV-2 infections. Herpes simplex virus type 2 (HSV-2) is the most common cause of genital herpes.
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